Characterization of Retinal Vascular and Neural Damage in a Novel Model of Diabetic Retinopathy.

نویسندگان

  • Lakshini Y Weerasekera
  • Lois A Balmer
  • Ramesh Ram
  • Grant Morahan
چکیده

PURPOSE Diabetic retinopathy (DR) is a major cause of blindness globally. Investigating the underlying mechanisms of DR would be aided by a suitable mouse model that developed key features seen in the human disease, and did so without carrying genetic modifications. This study was undertaken to produce such a model. METHODS Our panel of Collaborative Cross strains was screened for DR-like features after induction of diabetes by intravenous injection with alloxan or streptozotocin. Both flat-mounted whole-retina and histologic sections were studied for the presence of retinal lesions. Progression of DR was also studied by histologic examination of the retinal vascular and neural structure at various time points after diabetes onset. In addition, microarray investigations were conducted on retinas from control and diabetic mice. RESULTS Features of DR such as degenerated pericytes, acellular capillaries, minor vascular proliferation, gliosis of Müller cells, and loss of ganglion cells were noted as early as day 7 in some mice. These lesions became more evident with time. After 21 days of diabetes, severe vascular proliferation, microaneurysms, preretinal damage, increased Müller cell gliosis, and damage to the outer retina were all obvious. Microarray studies found significant differential expression of multiple genes known to be involved in DR. CONCLUSIONS The FOT_FB strain provides a useful model to investigate the pathogenesis of DR and to develop treatments for this vision-threatening disease.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Decrease of Serum Vascular Endothelial Growth Factor, along with its Ocular Level, after the Periocular Injection of Celecoxib and Propranolol in Streptozotocin-induced Diabetic Mouse Model

Background: There is a direct correlation between ocular vascular endothelial growth factor (VEGF) level and progression of pathological outcomes in diabetic retinopathy. In our previous study, the periocular administration of propranolol and celecoxib could significantly reduce ocular VEGF levels in a diabetic mouse model. Here, we investigated the changes of serum VEGF after ...

متن کامل

ارایه مدلی از شبکه‌های عصبی خودسازمان‌ده سلسله مراتبی در جهت تشخیص و طبقه‌بندی ضایعات شبکیه برای درجه‌بندی رتینوپاتی دیابتی

Background: One common symptom of diabetes is diabetic retinopathy, if not timely diagnosed and treated, leads to blindness. Retinal image analysis has been currently adopted to diagnose retinopathy. In this study, a model of hierarchical self-organized neural networks has been presented for the detection and classification of retina in diabetic patients. Methods: This study is a retrospective...

متن کامل

Prevention of leukostasis and vascular leakage in streptozotocin-induced diabetic retinopathy via intercellular adhesion molecule-1 inhibition.

Diabetic retinopathy is a leading cause of adult vision loss and blindness. Much of the retinal damage that characterizes the disease results from retinal vascular leakage and nonperfusion. This study shows that diabetic retinal vascular leakage and nonperfusion are temporally and spatially associated with retinal leukocyte stasis (leukostasis) in the rat model of streptozotocin-induced diabete...

متن کامل

Association between diabetic retinopathy and hemoglobin level

Background: Anemia may be considered to be an independent risk factor for the development of diabetic retinopathy (DR) in patients with renal failure. The purpose of this study was to investigate the association between blood hemoglobin level and retinopathy in diabetic patients with normal renal function tests.Methods: From 2009 to 2011, 1100 diabetic patients underwent retinal examination. Am...

متن کامل

مقایسه قدرت پیش بینی شبکه عصبی مصنوعی با رگرسیون لجستیک چندگانه در تفکیک بیماران دیابتی رتینوپاتی از غیر رتینوپاتی

 Background: Diabetes mellitus is a high prevalent disease among the population, and if not controlled, it causes complications and irreparable damage to the eye and cause blindness. This study goal is to investigate the predictive power of multiple logistic regression model and the Artificial Neural Network Multi-layer Perceptron (MLP) in determining patients with and without diabetic...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Investigative ophthalmology & visual science

دوره 56 6  شماره 

صفحات  -

تاریخ انتشار 2015